A little care. A new beginning.

Tissue culture acclimation. From flask to a place in your jungle.

Tissue culture plants grow in a sterile container at close to 100% humidity. Acclimation is the slow move from that container to normal room air: high humidity first, then gradually less, while new roots and leaves adapt. Here is how to do it, stage by stage, with the equipment and guides that help.

01Prepare

Prepare a humid space

Set up the space before the plants arrive. In an IKEA greenhouse such as ÅKERBÄR, water-filled trays raise the humidity while honeycomb grids keep pots above the water. Give them bright, indirect light and stable warmth, and keep the doors closed for the first days.

Greenhouse not included. Humidity depends on enclosure and ventilation.

Prepare a humid space

Prepare your growing space

02Deflask

Deflask and pot each plant

Rinse the agar off the roots with lukewarm water, as leftover agar feeds mould. Pot each plantlet in its own small cup with a light, airy medium, then cover it with a dome. A self-watering humidity cup keeps moisture even from below, so you rarely need to open the lid to water.

Pink pot with a clear humidity dome over a small variegated plantlet, with the text Acclimate your tissue cultures

Small plants. A space of their own.

04Grow

Pot up and grow on

When the plant grows confidently with the dome off, give it a bigger pot. A self-watering pot with a removable inner pot lets you check roots without disturbing them, and climbers can move onto a moss pole once they start reaching.

Explore all pots
Pot up and grow on

Settled in. Ready to grow on.

Common questions

How long does tissue culture acclimation take?

Usually two to six weeks, depending on the plant and your room humidity. Go by the plant, not the calendar: new leaves and roots mean it is ready for less humidity. The Complete Guide to Acclimating Tissue-Culture

Why are my tissue culture plants wilting?

Most often the humidity dropped too fast. Close the dome again for a few days, then open it more slowly. Wilting with a wet, dark base points to rot instead, so check the roots.

Do I need to remove all the agar?

Yes, as much as you can without tearing the roots. Agar is full of sugar and quickly grows mould outside the sterile container.

Should I keep plants separate or together?

Separate cups make it easy to spot problems early and stop rot or pests from spreading between plants.

Follow your plant supplier’s acclimation instructions.
Choose the equipment that suits your plants.